In order to generate GFP-Xf infected plants for microscopy, we are mechanically inoculating alternate hosts and grapes. Blue-green sharpshooters failed to efficiently inoculate wild-type Xf and green fluorescent protein-expressing (GFP) Xf into both grapes and alternate hosts only one of 44 grapes inoculated with BGSS became infected. Though alternate hosts had various vascular morphologies and stem lengths, all had shorter vessels than grapes. In all four alternate hosts, morning glory (Ipomoea purpurea), mugwort (Artemisia douglasiana), sunflower (Helianthus annuus) and annual bur-sage (Ambrosia acanthicarpa), the longest vessels measured were less than 13 cm long, while in grapes the longest vessels averaged 62 cm. The only significant difference was that tolerant Sylvaner had ~ 20 % more rays than the more susceptible Cabernet Sauvignon or Pinot Noir (n = 25, P = 0.01) in canes of similar age, length and diameter. Anatomical comparisons of three cultivars, Sylvaner, Cabernet Sauvignon and Pinot Noir showed that all three varieties had similar numbers, lengths and distributions of vessels.
We also address the short-term goal of controlling PD spread by comparing grape cultivars in their ability to provide inoculum for vine-to-vine spread of Pierces disease. Since host plant resistance is an important component in the long-term goal of curing PD, it is important to know how resistant plants affect PD spread in areas permanently infested with sharpshooter vectors. In this regard, remote sensing (RS) methods that respond to the plant traits most sensitive to Xf infection (e.g., Zarco-Tejada et al., 2018) could help to. Sap from the engineered plants successfully killed Xf in laboratory tests, and the. 'The hybrid protein apparently creates pores in the membrane of the Gram-negative bacterium, Xf,' said Gupta, thus allowing the plant to fight back the infection. We are observing Xf population and distribution in tolerant and susceptible plants, and its relationship to xylem anatomy, symptom development, and bacterial acquisition by sharpshooters. The team inserted the hybrid gene into grapevines and observed the plants response to Xf infection. coli O157:H7 host, but it plaques on a wide range of E. (poster from Miguel Roman et al., 2021 3rd EFSA European Conference) Experiment Strain Subspecie Isolated from Geographic origin ST AIVIA5901multiplex Prunus dulcisBollulla, Alicante (Spain) 6. LG1 was isolated from chicken feces on an E. no symptoms after more than 2 years on the inoculated olives. campestris results in an increase in this bacterium’s virulence towards rice (26,33). Abstract: We are studying the effect of host plant tolerance on insect vector acquisition of Xylella fastidiosa (Xf) from plants tolerant, moderately susceptible, and highly susceptible to Xf infection. Also of interest, phage Xf and Xf-2 infection of X.PATTERNS OF XYLELLA FASTIDIOSA INFECTION IN PLANTS AND EFFECTS ON ACQUISITION BY INSECT VECTORS
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